Vol. 3, Issue 3 (2018)
Estimation of antimicrobial activity and nano-toxicity with optimized ZnO nanoparticles
Author(s): Sakshi Chauhan, Saloni Singhal, Sandeep Sirohi
Abstract: The present study was concerned about the optimization and characterization (0.3M, 0.4M, 0.5M, 0.6M, 0.7M, 0.8M) of zinc oxide nanoparticles and their use as antibacterial agent. Chemical synthesized zinc oxide nanoparticles (precipitation) has been used. The synthesized zinc oxide nanoparticles were check by UV- VIS Spectrophotometer and characterized by Fourier transform infrared spectroscopy which has used for detection of functional group present in synthesized zinc oxide nanoparticles. The bacterial species used for examining of the antimicrobial activity of zinc oxide nanoparticles are Enterobacter aerogenes, Aeromonas hydrophila, Bacillus amyloliquefaciens, Micrococcus luteus. Similarly, the antibacterial activity of standard antibiotics (Gentamicin, Ciprofloxacin and Ampicillin) was tested against pathogens. Among all, 0.3M showed enhanced activity against all bacteria. Tox trak test was also analyzed on optimized ZnO nanoparticles to check the toxicity against human gut microflora microorganism and resulted that 0.3M is less toxic to human gut.