The purpose of the study was “Comparison of mental toughness and motivation between Bangladesh and Nepal kho-kho players”. The subject for this study was 12th South Asian Games 2016, which was organized by India in Guwahati (Assam). Total 30 (Thirty) male Kho-Kho players, 15 players of Bangladesh and 15 player of Nepal were (19-30 years of age) selected. The sports motivation scale (sms28) developed by Luc G. Pelletier, Michelle Fortier, and the Sports Mental Toughness Questionnaire developed by A Dr. Alan Goldberg (2012) were selected for this study. The selected variables were motivation and mental toughness. The obtained data were analyzed by applying independent‘t’ test in order to comparison of mental toughness and motivation differential between Bangladesh and Nepal Kho-Kho players. Further, the level of significance was set at 0.05 level of confidence. The finding of the study reveals that there was no significant difference was found in case of mental toughness and motivation between Bangladesh and Nepal kho-kho players may be due to the reason that the players were undergone with same duration and training, or having an equal amount of training and experience, they are equally motivated and mentally tough to win 12th South Asian Games.