International Journal of Advanced Research and Development

International Journal of Advanced Research and Development


International Journal of Advanced Research and Development
International Journal of Advanced Research and Development
Vol. 3, Issue 1 (2018)

Cross sectional studies on vitamin D receptor gene TaqI polymorphisms in healthy individuals from south Indian population


Sheebanancy K, Maheswari C, Usha Raja Nanthini A

Vitamin D interacts with its receptor to play an important role in calcium homeostasis by regulating bone cell growth and differentiation, intestinal calcium absorption and parathyroid hormone secretion. The gene encoding VDR is located on Chromosome 12cen-q12, it has multiple gene polymorphisms in exon 2 and 3’UTR region (SNPs). TaqI (rs731236) are adjacent restriction fragment length polymorphisms (RFLP) which is located in the region of intron 8/exon 9. The following study aimed to investigate the distribution of VDR TaqI in unrelated healthy individuals in south Indian. Mouth wash were collected from 108 unrelated normal individuals (Male 54 and Female 54) from Kodaikanal, Tamilnadu. The investigation was done in accordance with the ethical principles outlined by the Indian Council of Medical Research (ICMR) guidelines for medical research involving human subjects and informed consent was obtained from volunteers. Genotyping Taq I, 2000bp restriction site polymorphism was done using PCR-RFLP with specific primers to amplify the fragment from the isolated DNA as per. Genotype frequencies were calculated in EXCEL and Hardy Weinberg equilibrium was tested using chi square with VASSARSTAT. Out of the 108 subject analyzed for TaqI (rs 731236), the following genotypic frequencies were obtained: TT 21%, Tt 63%, tt 17%. The south Indian TaqI (rs 731236) allele and genotype frequencies are statistically significant and hence genetically different from those Asian population Chinese-Hong Kong, Thailand, Japanese-Okinawa and North Indian-lucknow, Sahariya tribe, Bhil tribe, Chatttisgrah tribe, North Central Muslims, North Central, South East, North Indian-USA. In this study no significant difference in the distribution of genotypes and alleles of TaqI (rs 731236) were found between South Indian individuals and the Caucasian American, Caucasian UK, Caucasian Portugal, White North Carolina (Medeiros 2002), Black Pennsylvania, Australia, North Carolina, White Minnesota, Austria Caucasian-France, Caucasian-Sweden, Greece. Thus these populations were similar to south Indian Populations. Allelic and genotypic heterogeneity at the VDR TaqI locus between different ethnic populations may lead to differences in the pathogenesis of diseases.
Download  |  Pages : 889-893
How to cite this article:
Sheebanancy K, Maheswari C, Usha Raja Nanthini A. Cross sectional studies on vitamin D receptor gene TaqI polymorphisms in healthy individuals from south Indian population. International Journal of Advanced Research and Development, Volume 3, Issue 1, 2018, Pages 889-893
International Journal of Advanced Research and Development International Journal of Advanced Research and Development